› 1bot

X-ray diffraction
3.05Å resolution

CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN ESCHERICHIA COLI GLYCEROL KINASE AND THE ALLOSTERIC REGULATOR FRUCTOSE 1,6-BISPHOSPHATE.

Released:
DOI: 10.2210/pdb1bot/pdb
PDB entry 1bot coloured by chain, front view.
PDB entry 1bot coloured by chain, side view.
PDB entry 1bot coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of a complex of Escherichia coli glycerol kinase and an allosteric effector fructose 1,6-bisphosphate.
Ormö M, Bystrom CE, Remington SJ
Biochemistry 37 16565-72 (1998)
PMID: 9843423

Function and Biology Details

Reaction catalysed: 
ATP + glycerol = ADP + sn-glycerol 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141309 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol kinase Chains: O, Z
Molecule details ›
Chains: O, Z
Length: 501 amino acids
Theoretical weight: 56.16 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6F3 (Residues: 2-502; Coverage: 100%)
Gene names: JW3897, b3926, glpK
Sequence domains:
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

2 bound ligands:
Ligand EPE 1 x EPE
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P41212
Unit cell:
a: 168.8Å b: 168.8Å c: 202.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 not available
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.
Deutscher et al. (2006)
25 other articles

2 mentions without citation

The FGGY carbohydrate kinase family: insights into the evolution of functional specificities.
Zhang et al. (2011)
1 more