› 1br1

X-ray diffraction
3.5Å resolution

SMOOTH MUSCLE MYOSIN MOTOR DOMAIN-ESSENTIAL LIGHT CHAIN COMPLEX WITH MGADP.ALF4 BOUND AT THE ACTIVE SITE

Released:
DOI: 10.2210/pdb1br1/pdb
PDB entry 1br1 coloured by chain, front view.
PDB entry 1br1 coloured by chain, side view.
PDB entry 1br1 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Crystal structure of a vertebrate smooth muscle myosin motor domain and its complex with the essential light chain: visualization of the pre-power stroke state.
Dominguez R, Freyzon Y, Trybus KM, Cohen C
Cell 94 559-71 (1998)
PMID: 9741621

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135957 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Myosin-11 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 820 amino acids
Theoretical weight: 93.7 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P10587 (Residues: 2-819; Coverage: 41%)
Gene name: MYH11
Sequence domains:
Structure domains:
Myosin light polypeptide 6 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 150 amino acids
Theoretical weight: 16.87 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P02607 (Residues: 2-151; Coverage: 99%)
Gene name: MYL6
Structure domains: EF-hand

Ligands and Environments

3 bound ligands:
Ligand MG 4 x MG
Ligand ALF 4 x ALF
Ligand ADP 4 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P1
Unit cell:
a: 95.32Å b: 144.66Å c: 147.29Å
α: 111.21° β: 106.1° γ: 92.58°
R-values:
R R work R free
0.227 0.227 0.305
Expression system: Spodoptera frugiperda

Quick links

Citations

88 review citations

The genetic basis for cardiomyopathy: from mutation identification to mechanistic paradigms.
Seidman et al. (2001)
87 more

39 mentions without citation

Membrane fission by dynamin: what we know and what we need to know.
Antonny et al. (2016)
38 more