› 1brx

X-ray diffraction
2.3Å resolution

BACTERIORHODOPSIN/LIPID COMPLEX

Released:
DOI: 10.2210/pdb1brx/pdb
PDB entry 1brx coloured by chain, front view.
PDB entry 1brx coloured by chain, side view.
PDB entry 1brx coloured by chain, top view.
Source organism: Halobacterium salinarum
Primary publication:
Proton transfer pathways in bacteriorhodopsin at 2.3 angstrom resolution.
Luecke H, Richter HT, Lanyi JK
Science 280 1934-7 (1998)
PMID: 9632391

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 26.8 KDa
Source organism: Halobacterium salinarum
Expression system: Halobacterium salinarum
UniProt:
  • Canonical: P02945 (Residues: 14-261; Coverage: 95%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

1 bound ligand:
Ligand RET 1 x RET
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P63
Unit cell:
a: 60.85Å b: 60.85Å c: 108.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 not available 0.298
Expression system: Halobacterium salinarum

Quick links

Citations

50 review citations

Membrane protein folding and stability: physical principles.
White et al. (1999)
49 more

6 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
5 more