› 1bto

X-ray diffraction
2Å resolution

HORSE LIVER ALCOHOL DEHYDROGENASE COMPLEXED TO NADH AND (1S,3R)3-BUTYLTHIOLANE 1-OXIDE

Released:
DOI: 10.2210/pdb1bto/pdb
PDB entry 1bto coloured by chain, front view.
PDB entry 1bto coloured by chain, side view.
PDB entry 1bto coloured by chain, top view.
Source organism: Equus caballus
Primary publication:
Flexibility of liver alcohol dehydrogenase in stereoselective binding of 3-butylthiolane 1-oxides.
Cho H, Ramaswamy S, Plapp BV
Biochemistry 36 382-9 (1997)
PMID: 9003191

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase E chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 374 amino acids
Theoretical weight: 39.85 KDa
Source organism: Equus caballus
UniProt:
  • Canonical: P00327 (Residues: 2-375; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
2 bound ligands:
Ligand ZN 8 x ZN
Ligand SSB 4 x SSB
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 49.93Å b: 180.2Å c: 86.8Å
α: 90° β: 106° γ: 90°
R-values:
R R work R free
0.212 0.19 0.23

Quick links

Citations

3 review citations

Role of sulfur chirality in the chemical processes of biology.
Bentley R. (2005)
2 more

4 mentions without citation

The x-ray crystal structure of lysine-2,3-aminomutase from Clostridium subterminale.
Lepore et al. (2005)
3 more