› 1byi

X-ray diffraction
0.97Å resolution

STRUCTURE OF APO-DETHIOBIOTIN SYNTHASE AT 0.97 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb1byi/pdb
PDB entry 1byi coloured by chain, front view.
PDB entry 1byi coloured by chain, side view.
PDB entry 1byi coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of dethiobiotin synthetase at 0.97 A resolution.
Sandalova T, Schneider G, Käck H, Lindqvist Y
Acta Crystallogr D Biol Crystallogr 55 610-24 (1999)
PMID: 10089457

Function and Biology Details

Reaction catalysed: 
ATP + 7,8-diaminononanoate + CO(2) = ADP + phosphate + dethiobiotin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent dethiobiotin synthetase BioD 1 Chain: A
Molecule details ›
Chain: A
Length: 224 amino acids
Theoretical weight: 24.03 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P13000 (Residues: 2-225; Coverage: 100%)
Gene names: JW0761, b0778, bioD1
Sequence domains: CobQ/CobB/MinD/ParA nucleotide binding domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: C2
Unit cell:
a: 72.447Å b: 47.812Å c: 61.202Å
α: 90° β: 107.24° γ: 90°
R-values:
R R work R free
0.116 not available not available

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Citations

2 review citations

Carbon-oxygen hydrogen bonding in biological structure and function.
Horowitz et al. (2012)
1 more

24 mentions without citation

Macromolecular ab initio phasing enforcing secondary and tertiary structure.
Millán et al. (2015)
23 more