› 1bys

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF NUC COMPLEXED WITH TUNGSTATE

Released:
DOI: 10.2210/pdb1bys/pdb
PDB entry 1bys coloured by chain, front view.
PDB entry 1bys coloured by chain, side view.
PDB entry 1bys coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
Crystal structure of a phospholipase D family member.
Stuckey JA, Dixon JE
Nat Struct Biol 6 278-84 (1999)
PMID: 10074947

Function and Biology Details

Reaction catalysed: 
A phosphatidylcholine + H(2)O = choline + a phosphatidate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181283 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
phospholipase D Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 17.18 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q79SE0 (Residues: 23-177; Coverage: 100%)
Gene names: G3248_004530, G4J11_004549, nuc
Sequence domains: PLD-like domain
Structure domains: Endonuclease Chain A

Ligands and Environments

1 bound ligand:
Ligand WO4 1 x WO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P43212
Unit cell:
a: 59.25Å b: 59.25Å c: 111.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.203 0.272
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

35 review citations

Structure and function of type II restriction endonucleases.
Pingoud et al. (2001)
34 more

8 mentions without citation

Phospholipase D: enzymology, functionality, and chemical modulation.
Selvy et al. (2011)
7 more