› 1c0a

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF THE E. COLI ASPARTYL-TRNA SYNTHETASE : TRNAASP : ASPARTYL-ADENYLATE COMPLEX

Released:
DOI: 10.2210/pdb1c0a/pdb
PDB entry 1c0a coloured by chain, front view.
PDB entry 1c0a coloured by chain, side view.
PDB entry 1c0a coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Synthesis of aspartyl-tRNA(Asp) in Escherichia coli--a snapshot of the second step.
Eiler S, Dock-Bregeon A, Moulinier L, Thierry JC, Moras D
EMBO J 18 6532-41 (1999)
PMID: 10562565

Function and Biology Details

Reaction catalysed: 
ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149467 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Aspartate--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 585 amino acids
Theoretical weight: 65.44 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P21889 (Residues: 1-585; Coverage: 99%)
Gene names: JW1855, aspS, b1866, tls
Sequence domains:
Structure domains:
ASPARTYL TRNA Chain: B
Molecule details ›
Chain: B
Length: 77 nucleotides
Theoretical weight: 25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Sequence domains: tRNA

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand AMP 1 x AMP
Ligand AMO 1 x AMO
6 modified residues:
Ligand 4SU 1 x 4SU
Ligand H2U 3 x H2U
Ligand QUO 1 x QUO
Ligand G7M 1 x G7M
Ligand 5MU 1 x 5MU
Ligand PSU 2 x PSU

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE D41A
Spacegroup: P43212
Unit cell:
a: 101.2Å b: 101.2Å c: 231.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.249
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Aminoacyl-tRNA synthesis.
Ibba et al. (2000)
5 more

40 mentions without citation

Personalized Medicine in Mitochondrial Health and Disease: Molecular Basis of Therapeutic Approaches Based on Nutritional Supplements and Their Analogs.
Tragni et al. (2022)
39 more