› 1c0m

X-ray diffraction
2.53Å resolution

CRYSTAL STRUCTURE OF RSV TWO-DOMAIN INTEGRASE

Released:
DOI: 10.2210/pdb1c0m/pdb
PDB entry 1c0m coloured by chain, front view.
PDB entry 1c0m coloured by chain, side view.
PDB entry 1c0m coloured by chain, top view.
Source organism: Rous sarcoma virus
Primary publication:
Crystal structure of an active two-domain derivative of Rous sarcoma virus integrase.
Yang ZN, Mueser TC, Bushman FD, Hyde CC
J Mol Biol 296 535-48 (2000)
PMID: 10669607

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136819 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Integrase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 238 amino acids
Theoretical weight: 26.6 KDa
Source organism: Rous sarcoma virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P03354 (Residues: 1329-1566; Coverage: 15%)
Gene name: gag-pol
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P1
Unit cell:
a: 55.752Å b: 66.369Å c: 76.67Å
α: 67.47° β: 78.61° γ: 90.18°
R-values:
R R work R free
0.216 0.216 0.279
Expression system: Escherichia coli

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Citations

6 review citations

Retroviral integrase inhibitors year 2000: update and perspectives.
Pommier et al. (2000)
5 more

11 mentions without citation

Structural biology of retroviral DNA integration.
Li et al. (2011)
10 more