› 1c0w

X-ray diffraction
3.2Å resolution

CRYSTAL STRUCTURE OF THE COBALT-ACTIVATED DIPHTHERIA TOXIN REPRESSOR-DNA COMPLEX REVEALS A METAL BINDING SH-LIKE DOMAIN

Released:
DOI: 10.2210/pdb1c0w/pdb
PDB entry 1c0w coloured by chain, front view.
PDB entry 1c0w coloured by chain, side view.
PDB entry 1c0w coloured by chain, top view.
Source organism: Corynebacterium diphtheriae
Primary publication:
Crystal structure of a cobalt-activated diphtheria toxin repressor-DNA complex reveals a metal-binding SH3-like domain.
Pohl E, Holmes RK, Hol WG
J Mol Biol 292 653-67 (1999)
PMID: 10497029

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113520 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Diphtheria toxin repressor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 225 amino acids
Theoretical weight: 25.22 KDa
Source organism: Corynebacterium diphtheriae
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DJL7 (Residues: 2-226; Coverage: 100%)
Gene names: DIP1414, dtxR
Sequence domains:
Structure domains:
DNA (5'-D(P*AP*TP*TP*AP*GP*GP*TP*TP*AP*GP*CP*CP*TP*AP*CP*CP*CP*TP*AP*AP*T)-3') Chain: E
Molecule details ›
Chain: E
Length: 21 nucleotides
Theoretical weight: 6.4 KDa
Source organism: Corynebacterium diphtheriae
Expression system: Not provided
DNA (5'-D(P*AP*TP*TP*AP*GP*GP*TP*TP*AP*GP*GP*CP*TP*AP*CP*CP*CP*TP*AP*AP*T)-3') Chain: F
Molecule details ›
Chain: F
Length: 21 nucleotides
Theoretical weight: 6.44 KDa
Source organism: Corynebacterium diphtheriae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CO 8 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P4222
Unit cell:
a: 180.2Å b: 180.2Å c: 151Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.249 0.279
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

8 review citations

Iron and metal regulation in bacteria.
Hantke K. (2001)
7 more

7 mentions without citation

Bacterial iron detoxification at the molecular level.
Bradley et al. (2020)
6 more