› 1c5f

X-ray diffraction
2.47Å resolution

CRYSTAL STRUCTURE OF THE CYCLOPHILIN-LIKE DOMAIN FROM BRUGIA MALAYI COMPLEXED WITH CYCLOSPORIN A

Released:
DOI: 10.2210/pdb1c5f/pdb
PDB entry 1c5f coloured by chain, front view.
PDB entry 1c5f coloured by chain, side view.
PDB entry 1c5f coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the complex of brugia malayi cyclophilin and cyclosporin A.
Ellis PJ, Carlow CK, Ma D, Kuhn P
Biochemistry 39 592-8 (2000)
PMID: 10642184

Function and Biology Details

Reaction catalysed: 
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162127 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peptidyl-prolyl cis-trans isomerase 1 Chains: A, C, E, G, I, K, M, O
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 177 amino acids
Theoretical weight: 19.5 KDa
Source organism: Brugia malayi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27450 (Residues: 1-177; Coverage: 21%)
Gene name: CYP-1
Sequence domains: Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Structure domains: Cyclophilin-like
CYCLOSPORIN A Chains: B, D, F, H, J, L, N, P
Molecule details ›
Chains: B, D, F, H, J, L, N, P
Length: 11 amino acids
Theoretical weight: 1.22 KDa
Source organism: Tolypocladium inflatum
Expression system: Not provided

Ligands and Environments

No bound ligands
6 modified residues:
Ligand DAL 8 x DAL
Ligand MLE 32 x MLE
Ligand MVA 8 x MVA
Ligand BMT 8 x BMT
Ligand ABA 8 x ABA
Ligand SAR 8 x SAR

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 62Å b: 100.16Å c: 133.92Å
α: 90° β: 93.75° γ: 90°
R-values:
R R work R free
0.201 0.201 0.249
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 citations in other articles

Structure-guided manipulation of the regioselectivity of the cyclosporine A hydroxylase CYP-sb21 from Sebekia benihana.
Li et al. (2020)