› 1c76

X-ray diffraction
2.25Å resolution

STAPHYLOKINASE (SAK) MONOMER

Released:
DOI: 10.2210/pdb1c76/pdb
PDB entry 1c76 coloured by chain, front view.
PDB entry 1c76 coloured by chain, side view.
PDB entry 1c76 coloured by chain, top view.
Source organism: Staphylococcus aureus
Entry authors: Rao Z, Jiang F, Liu Y, Zhang X, Chen Y, Bartlam M, Song H, Ding Y

Function and Biology Details

Reaction catalysed: 
Cleavage of insulin B chain with specificity similar to that of thermolysin, preferring hydrophobic P1' residue. Activates the glutamyl endopeptidase (EC 3.4.21.19) of Staphylococcus aureus.
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159507 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Staphylokinase Chain: A
Molecule details ›
Chain: A
Length: 136 amino acids
Theoretical weight: 15.49 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P68802 (Residues: 28-163; Coverage: 100%)
Gene name: sak
Sequence domains: Staphylokinase/Streptokinase family
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 43.92Å b: 59.84Å c: 102.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.201 0.273
Expression system: Escherichia coli

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