› 1c7q

X-ray diffraction
2.3Å resolution

THE CRYSTAL STRUCTURE OF PHOSPHOGLUCOSE ISOMERASE/AUTOCRINE MOTILITY FACTOR/NEUROLEUKIN COMPLEXED WITH ITS CARBOHYDRATE PHOSPHATE INHIBITORS AND ITS SUBSTRATE RECOGNITION MECHANISM

Released:
DOI: 10.2210/pdb1c7q/pdb
PDB entry 1c7q coloured by chain, front view.
PDB entry 1c7q coloured by chain, side view.
PDB entry 1c7q coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
The crystal structure of phosphoglucose isomerase/autocrine motility factor/neuroleukin complexed with its carbohydrate phosphate inhibitors suggests its substrate/receptor recognition.
Chou CC, Sun YJ, Meng M, Hsiao CD
J Biol Chem 275 23154-60 (2000)
PMID: 10770936

Function and Biology Details

Reaction catalysed: 
Alpha-D-glucose 6-phosphate = beta-D-fructofuranose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate isomerase 2 Chain: A
Molecule details ›
Chain: A
Length: 445 amino acids
Theoretical weight: 50.2 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P13376 (Residues: 1-445; Coverage: 100%)
Gene names: pgi2, pgiB
Sequence domains: Phosphoglucose isomerase
Structure domains: Glucose-6-phosphate isomerase like protein; domain 1

Ligands and Environments

1 bound ligand:
Ligand BE1 1 x BE1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: I222
Unit cell:
a: 74.94Å b: 93.64Å c: 171.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.172 0.242
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
4 more

2 mentions without citation

Crystal structure of Bacillus subtilis YckF: structural and functional evolution.
Sanishvili et al. (2004)
1 more