› 1c7s

X-ray diffraction
1.8Å resolution

BETA-N-ACETYLHEXOSAMINIDASE MUTANT D539A COMPLEXED WITH DI-N-ACETYL-BETA-D-GLUCOSAMINE (CHITOBIASE)

Released:
DOI: 10.2210/pdb1c7s/pdb
PDB entry 1c7s coloured by chain, front view.
PDB entry 1c7s coloured by chain, side view.
PDB entry 1c7s coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
Structures of chitobiase mutants complexed with the substrate Di-N-acetyl-d-glucosamine: the catalytic role of the conserved acidic pair, aspartate 539 and glutamate 540.
Prag G, Papanikolau Y, Tavlas G, Vorgias CE, Petratos K, Oppenheim AB
J Mol Biol 300 611-7 (2000)
PMID: 10884356

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176209 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Chitobiase Chain: A
Molecule details ›
Chain: A
Length: 858 amino acids
Theoretical weight: 95.94 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q54468 (Residues: 28-885; Coverage: 100%)
Gene name: chb
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21212
Unit cell:
a: 108.794Å b: 99.96Å c: 86.308Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.171 0.224
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Glycosidase mechanisms.
Vasella et al. (2002)
1 more

4 mentions without citation

Structure of the dimeric N-glycosylated form of fungal beta-N-acetylhexosaminidase revealed by computer modeling, vibrational spectroscopy, and biochemical studies.
Ettrich et al. (2007)
3 more