› 1c9w

X-ray diffraction
2.4Å resolution

CHO REDUCTASE WITH NADP+

Released:
DOI: 10.2210/pdb1c9w/pdb
PDB entry 1c9w coloured by chain, front view.
PDB entry 1c9w coloured by chain, side view.
PDB entry 1c9w coloured by chain, top view.
Source organism: Cricetulus griseus
Primary publication:
Crystal structure of CHO reductase, a member of the aldo-keto reductase superfamily.
Ye Q, Hyndman D, Li X, Flynn TG, Jia Z
Proteins 38 41-8 (2000)
PMID: 10651037

Function and Biology Details

Reaction catalysed: 
Alditol + NAD(P)(+) = aldose + NAD(P)H
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126670 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldose reductase-related protein 2 Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 36.26 KDa
Source organism: Cricetulus griseus
Expression system: Escherichia coli
UniProt:
  • Canonical: O08782 (Residues: 2-316; Coverage: 100%)
Gene name: AKR1B8
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3221
Unit cell:
a: 84.282Å b: 84.282Å c: 120.539Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.201 0.244
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

The crystal structure of rat liver AKR7A1. A dimeric member of the aldo-keto reductase superfamily.
Kozma et al. (2002)
6 more

1 mention without citation

Developing an effective polarizable bond method for small molecules with application to optimized molecular docking.
Duan et al. (2020)