› 1ca1

X-ray diffraction
1.9Å resolution

ALPHA-TOXIN FROM CLOSTRIDIUM PERFRINGENS

Released:
DOI: 10.2210/pdb1ca1/pdb
PDB entry 1ca1 coloured by chain, front view.
PDB entry 1ca1 coloured by chain, side view.
PDB entry 1ca1 coloured by chain, top view.
Source organism: Clostridium perfringens
Primary publication:
Structure of the key toxin in gas gangrene.
Naylor CE, Eaton JT, Howells A, Justin N, Moss DS, Titball RW, Basak AK
Nat Struct Biol 5 738-46 (1998)
PMID: 9699639

Function and Biology Details

Reaction catalysed: 
A phosphatidylcholine + H(2)O = 1,2-diacyl-sn-glycerol + phosphocholine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-142986 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase C Chain: A
Molecule details ›
Chain: A
Length: 370 amino acids
Theoretical weight: 42.62 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C216 (Residues: 29-398; Coverage: 100%)
Gene names: CPE0036, cpa, plc
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand CD 12 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.5
Spacegroup: C2221
Unit cell:
a: 61.3Å b: 177.3Å c: 79.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.254
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Clostridium perfringens: toxinotype and genotype.
Petit et al. (1999)
18 more

16 mentions without citation

Bacterial Sphingomyelinases and Phospholipases as Virulence Factors.
Flores-Díaz et al. (2016)
15 more