› 1cf5

X-ray diffraction
2.55Å resolution

BETA-MOMORCHARIN STRUCTURE AT 2.55 A

Released:
DOI: 10.2210/pdb1cf5/pdb
PDB entry 1cf5 coloured by chain, front view.
PDB entry 1cf5 coloured by chain, side view.
PDB entry 1cf5 coloured by chain, top view.
Source organism: Momordica charantia
Primary publication:
Three-dimensional structure of beta-momorcharin at 2.55 A resolution.
Yuan YR, He YN, Xiong JP, Xia ZX
Acta Crystallogr D Biol Crystallogr 55 1144-51 (1999)
PMID: 10329776

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150292 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Ribosome-inactivating protein beta-momorcharin Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 28.12 KDa
Source organism: Momordica charantia
UniProt:
  • Canonical: P24817 (Residues: 24-272; Coverage: 95%)
Gene names: MAP30, RIP
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, XYP, MAN, FUC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P1
Unit cell:
a: 49.09Å b: 50.58Å c: 61.12Å
α: 72.98° β: 78.39° γ: 76.97°
R-values:
R R work R free
0.172 0.172 0.278

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Citations

1 review citation

Biological activities of ribosome-inactivating proteins and their possible applications as antimicrobial, anticancer, and anti-pest agents and in neuroscience research.
Akkouh et al. (2015)
8 other articles

4 mentions without citation

Strategies to Improve the Clinical Utility of Saporin-Based Targeted Toxins.
Giansanti et al. (2018)
3 more