› 1ch8

X-ray diffraction
2.5Å resolution

STRUCTURE OF ADENYLOSUCCINATE SYNTHETASE FROM E. COLI COMPLEXED WITH A STRINGENT EFFECTOR, PPG2':3'P

Released:
DOI: 10.2210/pdb1ch8/pdb
PDB entry 1ch8 coloured by chain, front view.
PDB entry 1ch8 coloured by chain, side view.
PDB entry 1ch8 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Effectors of the stringent response target the active site of Escherichia coli adenylosuccinate synthetase.
Hou Z, Cashel M, Fromm HJ, Honzatko RB
J Biol Chem 274 17505-10 (1999)
PMID: 10364182

Function and Biology Details

Reaction catalysed: 
GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-141583 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate synthetase Chain: A
Molecule details ›
Chain: A
Length: 431 amino acids
Theoretical weight: 47.27 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7D4 (Residues: 2-432; Coverage: 100%)
Gene names: JW4135, adeK, b4177, purA
Sequence domains: Adenylosuccinate synthetase
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand NO3 1 x NO3
Ligand MG 1 x MG
Ligand GPX 1 x GPX
Ligand HDA 1 x HDA
Ligand IMP 1 x IMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P3221
Unit cell:
a: 80.3Å b: 80.3Å c: 158.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.19 0.25
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Revisiting the stringent response, ppGpp and starvation signaling.
Chatterji et al. (2001)
6 more

2 mentions without citation

Novel (p)ppGpp Binding and Metabolizing Proteins of Escherichia coli.
Zhang et al. (2018)
1 more