› 1chk

X-ray diffraction
2.4Å resolution

STREPTOMYCES N174 CHITOSANASE PH5.5 298K

Released:
DOI: 10.2210/pdb1chk/pdb
PDB entry 1chk coloured by chain, front view.
PDB entry 1chk coloured by chain, side view.
PDB entry 1chk coloured by chain, top view.
Source organism: Streptomyces sp. N174
Primary publication:
X-ray structure of an anti-fungal chitosanase from streptomyces N174.
Marcotte EM, Monzingo AF, Ernst SR, Brzezinski R, Robertus JD
Nat Struct Biol 3 155-62 (1996)
PMID: 8564542

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of beta-(1->4)-linkages between D-glucosamine residues in a partly acetylated chitosan
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152630 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chitosanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 238 amino acids
Theoretical weight: 25.85 KDa
Source organism: Streptomyces sp. N174
Expression system: Streptomyces lividans
UniProt:
  • Canonical: P33665 (Residues: 41-278; Coverage: 100%)
Gene names: chs, csn
Sequence domains: Glycosyl hydrolase family 46
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 56.3Å b: 59.3Å c: 85.4Å
α: 90° β: 96.2° γ: 90°
R-values:
R R work R free
0.181 0.181 0.175
Expression system: Streptomyces lividans

Quick links

Citations

14 review citations

Structural and sequence-based classification of glycoside hydrolases.
Henrissat et al. (1997)
13 more

9 mentions without citation

Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes.
Viens et al. (2015)
8 more