› 1cl0

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF REDUCED THIOREDOXIN REDUCTASE FROM ESCHERICHIA COLI.

Released:
DOI: 10.2210/pdb1cl0/pdb
PDB entry 1cl0 coloured by chain, front view.
PDB entry 1cl0 coloured by chain, side view.
PDB entry 1cl0 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of reduced thioredoxin reductase from Escherichia coli: structural flexibility in the isoalloxazine ring of the flavin adenine dinucleotide cofactor.
Lennon BW, Williams CH, Ludwig ML
Protein Sci 8 2366-79 (1999)
PMID: 10595539

Function and Biology Details

Reaction catalysed: 
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141909 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin reductase Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 34.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A9P4 (Residues: 2-321; Coverage: 100%)
Gene names: JW0871, b0888, trxB
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P6322
Unit cell:
a: 121.298Å b: 121.298Å c: 81.32Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.2 0.292
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Sequence-structure analysis of FAD-containing proteins.
Dym et al. (2001)
6 more

9 mentions without citation

Protein-protein docking benchmark version 4.0.
Hwang et al. (2010)
8 more