› 1cnu

X-ray diffraction
2.25Å resolution

PHOSPHORYLATED ACTOPHORIN FROM ACANTAMOEBA POLYPHAGA

Released:
DOI: 10.2210/pdb1cnu/pdb
PDB entry 1cnu coloured by chain, front view.
PDB entry 1cnu coloured by chain, side view.
PDB entry 1cnu coloured by chain, top view.
Source organism: Acanthamoeba polyphaga
Primary publication:
Interaction of actin monomers with Acanthamoeba actophorin (ADF/cofilin) and profilin.
Blanchoin L, Pollard TD
J Biol Chem 273 25106-11 (1998)
PMID: 9737968

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153363 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actophorin Chain: A
Molecule details ›
Chain: A
Length: 137 amino acids
Theoretical weight: 15.52 KDa
Source organism: Acanthamoeba polyphaga
Expression system: Escherichia coli
UniProt:
  • Canonical: P37167 (Residues: 2-138; Coverage: 99%)
Sequence domains: Cofilin/tropomyosin-type actin-binding protein
Structure domains: Severin

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE M18X
Spacegroup: P212121
Unit cell:
a: 40.2Å b: 46.5Å c: 68.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.304
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Molecular mechanisms controlling actin filament dynamics in nonmuscle cells.
Pollard et al. (2000)
20 more

9 mentions without citation

The ADF/cofilin family: actin-remodeling proteins.
Maciver et al. (2002)
8 more