› 1cpm

X-ray diffraction
2Å resolution

NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS

Released:
DOI: 10.2210/pdb1cpm/pdb
PDB entry 1cpm coloured by chain, front view.
PDB entry 1cpm coloured by chain, side view.
PDB entry 1cpm coloured by chain, top view.
Source organism: Paenibacillus macerans
Primary publication:
Native-like in vivo folding of a circularly permuted jellyroll protein shown by crystal structure analysis.
Hahn M, Piotukh K, Borriss R, Heinemann U
Proc Natl Acad Sci U S A 91 10417-21 (1994)
PMID: 7937966

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150105 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucanase Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 23.88 KDa
Source organism: Paenibacillus macerans
Expression system: Not provided
UniProt:
  • Canonical: P23904 (Residues: 82-237; Coverage: 73%)
Sequence domains: Glycosyl hydrolases family 16
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 39.43Å b: 44.3Å c: 128.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.153 not available
Expression system: Not provided

Quick links

Citations

4 review citations

Bacterial 1,3-1,4-beta-glucanases: structure, function and protein engineering.
Planas A. (2000)
3 more

1 mention without citation

CPSARST: an efficient circular permutation search tool applied to the detection of novel protein structural relationships.
Lo et al. (2008)