› 1crl

X-ray diffraction
2.06Å resolution

INSIGHTS INTO INTERFACIAL ACTIVATION FROM AN 'OPEN' STRUCTURE OF CANDIDA RUGOSA LIPASE

Released:
DOI: 10.2210/pdb1crl/pdb
PDB entry 1crl coloured by chain, front view.
PDB entry 1crl coloured by chain, side view.
PDB entry 1crl coloured by chain, top view.
Source organism: Diutina rugosa
Primary publication:
Insights into interfacial activation from an open structure of Candida rugosa lipase.
Grochulski P, Li Y, Schrag JD, Bouthillier F, Smith P, Harrison D, Rubin B, Cygler M
J Biol Chem 268 12843-7 (1993)
PMID: 8509417

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148911 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lipase 1 Chain: A
Molecule details ›
Chain: A
Length: 534 amino acids
Theoretical weight: 57.13 KDa
Source organism: Diutina rugosa
Expression system: Not provided
UniProt:
  • Canonical: P20261 (Residues: 16-549; Coverage: 100%)
Gene name: LIP1
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 64.9Å b: 97.5Å c: 175.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.134 0.134 not available
Expression system: Not provided

Quick links

Citations

27 review citations

Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases.
Jaeger et al. (1999)
26 more

23 mentions without citation

Tools for integrated sequence-structure analysis with UCSF Chimera.
Meng et al. (2006)
22 more