› 1cu1

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF AN ENZYME COMPLEX FROM HEPATITIS C VIRUS

Released:
DOI: 10.2210/pdb1cu1/pdb
PDB entry 1cu1 coloured by chain, front view.
PDB entry 1cu1 coloured by chain, side view.
PDB entry 1cu1 coloured by chain, top view.
Source organism: Hepacivirus hominis
Primary publication:
Molecular views of viral polyprotein processing revealed by the crystal structure of the hepatitis C virus bifunctional protease-helicase.
Yao N, Reichert P, Taremi SS, Prosise WW, Weber PC
Structure 7 1353-63 (1999)
PMID: 10574797

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150815 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease/helicase NS3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 645 amino acids
Theoretical weight: 68.57 KDa
Source organism: Hepacivirus hominis
Expression system: Escherichia coli
UniProt:
  • Canonical: P26663 (Residues: 1678-1657; Coverage: 21%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS
Spacegroup: P212121
Unit cell:
a: 91.36Å b: 110.51Å c: 141.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.26
Expression system: Escherichia coli

Quick links

Citations

45 review citations

SF1 and SF2 helicases: family matters.
Fairman-Williams et al. (2010)
44 more

59 mentions without citation

Viral and cellular RNA helicases as antiviral targets.
Kwong et al. (2005)
58 more