1cv8

X-ray diffraction
1.75Å resolution

STAPHOPAIN, CYSTEINE PROTEINASE FROM STAPHYLOCOCCUS AUREUS V8

Released:
Source organism: Staphylococcus aureus

Function and Biology Details

Reaction catalysed:
Broad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160505 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Staphopain A Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 19.87 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: P81297 (Residues: 215-388; Coverage: 48%)
Gene names: scpA, sspP
Sequence domains: Staphopain peptidase C47
Structure domains: Cysteine proteinases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P6522
Unit cell:
a: 61.139Å b: 61.139Å c: 195.063Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.2 0.239