› 1cvl

X-ray diffraction
1.6Å resolution

CRYSTAL STRUCTURE OF BACTERIAL LIPASE FROM CHROMOBACTERIUM VISCOSUM ATCC 6918

Released:
DOI: 10.2210/pdb1cvl/pdb
PDB entry 1cvl coloured by chain, front view.
PDB entry 1cvl coloured by chain, side view.
PDB entry 1cvl coloured by chain, top view.
Source organism: Chromobacterium viscosum
Primary publication:
Crystal structure of a bacterial lipase from Chromobacterium viscosum ATCC 6918 refined at 1.6 angstroms resolution.
Lang D, Hofmann B, Haalck L, Hecht HJ, Spener F, Schmid RD, Schomburg D
J Mol Biol 259 704-17 (1996)
PMID: 8683577

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145129 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triacylglycerol lipase Chain: A
Molecule details ›
Chain: A
Length: 319 amino acids
Theoretical weight: 33.12 KDa
Source organism: Chromobacterium viscosum
UniProt:
  • Canonical: P0DUB9 (Residues: 1-319; Coverage: 100%)
Gene names: lip, lipA
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P21212
Unit cell:
a: 41.08Å b: 156.82Å c: 43.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 not available

Quick links

Citations

12 review citations

Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases.
Jaeger et al. (1999)
11 more

14 mentions without citation

Carboxylic ester hydrolases: Classification and database derived from their primary, secondary, and tertiary structures.
Chen et al. (2016)
13 more