› 1cw9

X-ray diffraction
1.55Å resolution

DNA DECAMER WITH AN ENGINEERED CROSSLINK IN THE MINOR GROOVE

Released:
DOI: 10.2210/pdb1cw9/pdb
PDB entry 1cw9 coloured by chain, front view.
PDB entry 1cw9 coloured by chain, side view.
PDB entry 1cw9 coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
A structural snapshot of base-pair opening in DNA.
van Aalten DM, Erlanson DA, Verdine GL, Joshua-Tor L
Proc Natl Acad Sci U S A 96 11809-14 (1999)
PMID: 10518532

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113562 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
5'-D(*CP*CP*AP*GP*(G47)P*CP*CP*TP*GP*G)-3' Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 10 nucleotides
Theoretical weight: 3.11 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
1 modified residue:
Ligand G47 4 x G47

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P212121
Unit cell:
a: 34.435Å b: 43.18Å c: 72.412Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.203 0.232
Expression system: Not provided

Quick links

Citations

1 review citation

Atomistic view of base flipping in DNA.
Huang et al. (2004)
8 other articles

4 mentions without citation

DNA-directed base pair opening.
Timsit Y. (2012)
3 more