1cy4

X-ray diffraction
2.55Å resolution

COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH 5'pTpTpTp3'

Released:
Source organism: Escherichia coli K-12
Primary publication:
Protein-nucleotide interactions in E. coli DNA topoisomerase I.
Nat Struct Biol 6 961-8 (1999)
PMID: 10504732

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139094 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 1 Chain: A
Molecule details ›
Chain: A
Length: 599 amino acids
Theoretical weight: 67.54 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P06612 (Residues: 1-597; Coverage: 69%)
Gene names: JW1266, b1274, supX, topA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 63.95Å b: 78.76Å c: 141.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.221 0.299
Expression system: Escherichia coli