› 1cz8

X-ray diffraction
2.4Å resolution

VASCULAR ENDOTHELIAL GROWTH FACTOR IN COMPLEX WITH AN AFFINITY MATURED ANTIBODY

Released:
DOI: 10.2210/pdb1cz8/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1cz8 coloured by chain, front view.
PDB entry 1cz8 coloured by chain, side view.
PDB entry 1cz8 coloured by chain, top view.
Source organisms:
Primary publication:
Selection and analysis of an optimized anti-VEGF antibody: crystal structure of an affinity-matured Fab in complex with antigen.
Chen Y, Wiesmann C, Fuh G, Li B, Christinger HW, McKay P, de Vos AM, Lowman HB
J Mol Biol 293 865-81 (1999)
PMID: 10543973

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210642 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
N-VEGF Chains: V, W
Molecule details ›
Chains: V, W
Length: 94 amino acids
Theoretical weight: 11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15692 (Residues: 220-313; Coverage: 24%)
Gene names: VEGF, VEGFA
Sequence domains: PDGF/VEGF domain
Structure domains: Cystine-knot cytokines
LIGHT CHAIN OF NEUTRALIZING ANTIBODY Chains: L, X
Molecule details ›
Chains: L, X
Length: 214 amino acids
Theoretical weight: 23.45 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
HEAVY CHAIN OF NEUTRALIZING ANTIBODY Chains: H, Y

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 89.12Å b: 66.4Å c: 138.75Å
α: 90° β: 94.7° γ: 90°
R-values:
R R work R free
0.208 0.208 0.267
Expression system: Escherichia coli

Quick links

Citations

78 review citations

The biology of VEGF and its receptors.
Ferrara et al. (2003)
77 more

25 mentions without citation

Tying the knot: the cystine signature and molecular-recognition processes of the vascular endothelial growth factor family of angiogenic cytokines.
Iyer et al. (2011)
24 more