› 1czb

X-ray diffraction
1.06Å resolution

ATOMIC RESOLUTION ASV INTEGRASE CORE DOMAIN FROM HEPES

Released:
DOI: 10.2210/pdb1czb/pdb
PDB entry 1czb coloured by chain, front view.
PDB entry 1czb coloured by chain, side view.
PDB entry 1czb coloured by chain, top view.
Source organism: Avian sarcoma virus
Primary publication:
Atomic resolution structures of the core domain of avian sarcoma virus integrase and its D64N mutant.
Lubkowski J, Dauter Z, Yang F, Alexandratos J, Merkel G, Skalka AM, Wlodawer A
Biochemistry 38 13512-22 (1999)
PMID: 10521258

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131654 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Integrase Chain: A
Molecule details ›
Chain: A
Length: 162 amino acids
Theoretical weight: 17.86 KDa
Source organism: Avian sarcoma virus
Expression system: Escherichia coli
UniProt:
  • Canonical: O92956 (Residues: 1332-1487; Coverage: 10%)
Gene name: gag-pol
Sequence domains: Integrase core domain
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

1 bound ligand:
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P43212
Unit cell:
a: 65.37Å b: 65.37Å c: 80.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.143 0.164
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Piecing together the structure of retroviral integrase, an important target in AIDS therapy.
Jaskolski et al. (2009)
3 more

3 mentions without citation

Structural biology of retroviral DNA integration.
Li et al. (2011)
2 more