Function and Biology Details
Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
Structure domain:
Structure analysis Details
Assembly composition:
hetero tetramer (preferred)
Assembly name:
Type II restriction enzyme MunI and DNA (preferred)
PDBe Complex ID:
PDB-CPX-113568 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Type II restriction enzyme MunI
Molecule details ›
Chains: A, B
Length: 202 amino acids
Theoretical weight: 23.38 KDa
Source organism: Mycoplasma
Expression system: Escherichia coli
UniProt:
Sequence domains: Type II restriction enzyme MunI
Structure domains: Eco RI Endonuclease, subunit A
Length: 202 amino acids
Theoretical weight: 23.38 KDa
Source organism: Mycoplasma
Expression system: Escherichia coli
UniProt:
- Canonical:
P43642 (Residues: 1-202; Coverage: 100%)
Sequence domains: Type II restriction enzyme MunI
Structure domains: Eco RI Endonuclease, subunit A
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup:
P212121
Expression systems:
- Escherichia coli
- Not provided
