› 1d04

X-ray diffraction
1.85Å resolution

COMPARISONS OF WILD TYPE AND MUTANT FLAVODOXINS FROM ANACYSTIS NIDULANS. STRUCTURAL DETERMINANTS OF THE REDOX POTENTIALS.

Released:
DOI: 10.2210/pdb1d04/pdb
PDB entry 1d04 coloured by chain, front view.
PDB entry 1d04 coloured by chain, side view.
PDB entry 1d04 coloured by chain, top view.
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Primary publication:
Comparisons of wild-type and mutant flavodoxins from Anacystis nidulans. Structural determinants of the redox potentials.
Hoover DM, Drennan CL, Metzger AL, Osborne C, Weber CH, Pattridge KA, Ludwig ML
J Mol Biol 294 725-43 (1999)
PMID: 10610792

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145280 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavodoxin Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 18.66 KDa
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Expression system: Escherichia coli
UniProt:
  • Canonical: P10340 (Residues: 2-170; Coverage: 99%)
Gene names: Synpcc7942_1541, isiB
Sequence domains: Flavodoxin
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand FMN 1 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 57.18Å b: 69.4Å c: 46.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.168 0.207
Expression system: Escherichia coli

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Citations

7 review citations

Prokaryotic Heme Biosynthesis: Multiple Pathways to a Common Essential Product.
Dailey et al. (2017)
6 more