› 1d0q

X-ray diffraction
1.71Å resolution

STRUCTURE OF THE ZINC-BINDING DOMAIN OF BACILLUS STEAROTHERMOPHILUS DNA PRIMASE

Released:
DOI: 10.2210/pdb1d0q/pdb
PDB entry 1d0q coloured by chain, front view.
PDB entry 1d0q coloured by chain, side view.
PDB entry 1d0q coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Structure of the zinc-binding domain of Bacillus stearothermophilus DNA primase.
Pan H, Wigley DB
Structure 8 231-9 (2000)
PMID: 10745010

Function and Biology Details

Reaction catalysed: 
SsDNA + n NTP = ssDNA/pppN(pN)(n-1) hybrid + (n-1) diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194914 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA primase Chains: A, B
Molecule details ›
Chains: A, B
Length: 103 amino acids
Theoretical weight: 11.38 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Geobacillus stearothermophilus
UniProt:
  • Canonical: Q9X4D0 (Residues: 1-103; Coverage: 17%)
Gene name: dnaG
Sequence domains: CHC2 zinc finger
Structure domains: Zinc finger, CHC2-type domain

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.5, RIGAKU
Spacegroup: P21
Unit cell:
a: 36.17Å b: 58.745Å c: 45.75Å
α: 90° β: 91.73° γ: 90°
R-values:
R R work R free
0.204 0.204 0.225
Expression system: Geobacillus stearothermophilus

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Citations

6 review citations

Nitrosylation. the prototypic redox-based signaling mechanism.
Stamler et al. (2001)
5 more

11 mentions without citation

Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target.
Robinson et al. (2012)
10 more