› 1d1l

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF CRO-F58W MUTANT

Released:
DOI: 10.2210/pdb1d1l/pdb
PDB entry 1d1l coloured by chain, front view.
PDB entry 1d1l coloured by chain, side view.
PDB entry 1d1l coloured by chain, top view.
Source organism: Escherichia virus Lambda
Primary publication:
The structural basis for enhanced stability and reduced DNA binding seen in engineered second-generation Cro monomers and dimers.
Rupert PB, Mollah AK, Mossing MC, Matthews BW
J Mol Biol 296 1079-90 (2000)
PMID: 10686105

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-136477 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein cro Chain: A
Molecule details ›
Chain: A
Length: 61 amino acids
Theoretical weight: 6.88 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli
UniProt:
  • Canonical: P03040 (Residues: 1-61; Coverage: 92%)
Gene names: cro, lambdap57
Sequence domains: Cro
Structure domains: CRO Repressor

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: F222
Unit cell:
a: 59.86Å b: 67.3Å c: 91.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 0.258
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Role of loops connecting secondary structure elements in the stabilization of proteins isolated from thermophilic organisms.
Balasco et al. (2013)
4 more

4 mentions without citation

Conservation of the three-dimensional structure in non-homologous or unrelated proteins.
Sousounis et al. (2012)
3 more