› 1d1t

X-ray diffraction
2.4Å resolution

MUTANT OF HUMAN SIGMA ALCOHOL DEHYDROGENASE WITH LEUCINE AT POSITION 141

Released:
DOI: 10.2210/pdb1d1t/pdb
PDB entry 1d1t coloured by chain, front view.
PDB entry 1d1t coloured by chain, side view.
PDB entry 1d1t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Methionine-141 directly influences the binding of 4-methylpyrazole in human sigma sigma alcohol dehydrogenase.
Xie PT, Hurley TD
Protein Sci 8 2639-44 (1999)
PMID: 10631979

Function and Biology Details

Reactions catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
All-trans-retinol-[cellular-retinol-binding-protein] + NAD(+) = all-trans-retinal-[cellular-retinol-binding-protein] + NADH
10-hydroxydecanoate + NAD(+) = 10-oxodecanoate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154107 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
All-trans-retinol dehydrogenase [NAD(+)] ADH7 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 373 amino acids
Theoretical weight: 39.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P40394 (Residues: 14-386; Coverage: 97%)
Gene name: ADH7
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
3 bound ligands:
Ligand ZN 19 x ZN
Ligand ACT 13 x ACT
Ligand CAC 5 x CAC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 85.9Å b: 90.5Å c: 119.8Å
α: 90° β: 99.3° γ: 90°
R-values:
R R work R free
0.221 0.216 0.274
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Targeting Metalloenzymes for Therapeutic Intervention.
Chen et al. (2019)
3 more

5 mentions without citation

Plumbagin elicits differential proteomic responses mainly involving cell cycle, apoptosis, autophagy, and epithelial-to-mesenchymal transition pathways in human prostate cancer PC-3 and DU145 cells.
Qiu et al. (2015)
4 more