› 1d2k

X-ray diffraction
2.2Å resolution

C. IMMITIS CHITINASE 1 AT 2.2 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb1d2k/pdb
PDB entry 1d2k coloured by chain, front view.
PDB entry 1d2k coloured by chain, side view.
PDB entry 1d2k coloured by chain, top view.
Source organism: Coccidioides immitis
Primary publication:
The X-ray structure of a chitinase from the pathogenic fungus Coccidioides immitis.
Hollis T, Monzingo AF, Bortone K, Ernst S, Cox R, Robertus JD
Protein Sci 9 544-51 (2000)
PMID: 10752616

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endochitinase 1 Chain: A
Molecule details ›
Chain: A
Length: 392 amino acids
Theoretical weight: 43.71 KDa
Source organism: Coccidioides immitis
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CB51 (Residues: 36-427; Coverage: 96%)
Gene names: CPSG_08657, CTS1
Sequence domains: Glycosyl hydrolases family 18
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 91.248Å b: 91.248Å c: 95.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.17 0.248
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Review of fungal chitinases.
Duo-Chuan L. (2006)
2 more

8 mentions without citation

Sequence and structural analysis of the chitinase insertion domain reveals two conserved motifs involved in chitin-binding.
Li et al. (2010)
7 more