› 1d2n

X-ray diffraction
1.75Å resolution

D2 DOMAIN OF N-ETHYLMALEIMIDE-SENSITIVE FUSION PROTEIN

Released:
DOI: 10.2210/pdb1d2n/pdb
PDB entry 1d2n coloured by chain, front view.
PDB entry 1d2n coloured by chain, side view.
PDB entry 1d2n coloured by chain, top view.
Source organism: Cricetulus griseus
Primary publication:
Crystal structure of the hexamerization domain of N-ethylmaleimide-sensitive fusion protein.
Lenzen CU, Steinmann D, Whiteheart SW, Weis WI
Cell 94 525-36 (1998)
PMID: 9727495

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148437 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vesicle-fusing ATPase Chain: A
Molecule details ›
Chain: A
Length: 272 amino acids
Theoretical weight: 30.54 KDa
Source organism: Cricetulus griseus
Expression system: Escherichia coli
UniProt:
  • Canonical: P18708 (Residues: 479-743; Coverage: 36%)
Gene name: NSF
Sequence domains: ATPase family associated with various cellular activities (AAA)
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand ANP 1 x ANP
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P6
Unit cell:
a: 116.33Å b: 116.33Å c: 40.24Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.199 0.223
Expression system: Escherichia coli

Quick links

Citations

62 review citations

The 26S proteasome: a molecular machine designed for controlled proteolysis.
Voges et al. (1999)
61 more

18 mentions without citation

Cellular functions of NSF: not just SNAPs and SNAREs.
Zhao et al. (2007)
17 more