› 1d44

X-ray diffraction
2Å resolution

DNA DODECAMER C-G-C-G-A-A-T-T-C-G-C-G/HOECHST 33258 COMPLEX: 0 DEGREES C, PIPERAZINE DOWN

Released:
DOI: 10.2210/pdb1d44/pdb
PDB entry 1d44 coloured by chain, front view.
PDB entry 1d44 coloured by chain, side view.
PDB entry 1d44 coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Low-temperature crystallographic analyses of the binding of Hoechst 33258 to the double-helical DNA dodecamer C-G-C-G-A-A-T-T-C-G-C-G.
Quintana JR, Lipanov AA, Dickerson RE
Biochemistry 30 10294-306 (1991)
PMID: 1718416

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113607 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
DNA (5'-D(*CP*GP*CP*GP*AP*AP*TP*TP*CP*GP*CP*G)-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand HT 1 x HT
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 23.95Å b: 38.99Å c: 65.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 not available not available
Expression system: Not provided

Quick links

Citations

5 review citations

Energetics of drug-DNA interactions.
Chaires JB. (1997)
4 more

2 mentions without citation

Hydration changes in the association of Hoechst 33258 with DNA.
Kiser et al. (2005)
1 more