› 1d6h

X-ray diffraction
2.15Å resolution

CHALONE SYNTHASE (N336A MUTANT COMPLEXED WITH COA)

Released:
DOI: 10.2210/pdb1d6h/pdb
PDB entry 1d6h coloured by chain, front view.
PDB entry 1d6h coloured by chain, side view.
PDB entry 1d6h coloured by chain, top view.
Source organism: Medicago sativa
Primary publication:
Dissection of malonyl-coenzyme A decarboxylation from polyketide formation in the reaction mechanism of a plant polyketide synthase.
Jez JM, Ferrer JL, Bowman ME, Dixon RA, Noel JP
Biochemistry 39 890-902 (2000)
PMID: 10653632

Function and Biology Details

Reaction catalysed: 
3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151628 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chalcone synthase 2 Chain: A
Molecule details ›
Chain: A
Length: 387 amino acids
Theoretical weight: 42.51 KDa
Source organism: Medicago sativa
Expression system: Escherichia coli
UniProt:
  • Canonical: P30074 (Residues: 3-389; Coverage: 100%)
Gene name: CHS2
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments


Cofactor: Ligand COA 1 x COA
1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand CSD 1 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE
Spacegroup: P3221
Unit cell:
a: 97.361Å b: 97.361Å c: 65.207Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.184 0.257
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Metabolic channeling in plants.
Winkel BS. (2004)
14 more

2 mentions without citation

Structure and function of enzymes involved in the biosynthesis of phenylpropanoids.
Ferrer et al. (2008)
1 more