› 1d7l

X-ray diffraction
2.2Å resolution

STRUCTURE-FUNCTION CORRELATIONS OF THE REACTION OF REDUCED NICOTINAMIDE ANALOGS WITH P-HYDROXYBENZOATE HYDROXYLASE SUBSTITUTED WITH A SERIES OF 8-SUBSTITUTED FLAVINS

Released:
DOI: 10.2210/pdb1d7l/pdb
PDB entry 1d7l coloured by chain, front view.
PDB entry 1d7l coloured by chain, side view.
PDB entry 1d7l coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structure-function correlations of the reaction of reduced nicotinamide analogues with p-hydroxybenzoate hydroxylase substituted with a series of 8-substituted flavins.
Ortiz-Maldonado M, Gatti D, Ballou DP, Massey V
Biochemistry 38 16636-47 (1999)
PMID: 10600126

Function and Biology Details

Reaction catalysed: 
4-hydroxybenzoate + NADPH + O(2) = protocatechuate + NADP(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-149006 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
p-hydroxybenzoate hydroxylase Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 44.38 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P20586 (Residues: 1-394; Coverage: 100%)
Gene names: PA0247, pobA
Sequence domains: FAD binding domain
Structure domains:

Ligands and Environments


Cofactor: Ligand RFL 1 x RFL
2 bound ligands:
Ligand SO4 3 x SO4
Ligand PHB 1 x PHB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 70.802Å b: 145.477Å c: 86.966Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.184 0.235
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Protein dynamics and electrostatics in the function of p-hydroxybenzoate hydroxylase.
Entsch et al. (2005)
2 more

6 mentions without citation

From the similarity analysis of protein cavities to the functional classification of protein families using cavbase.
Kuhn et al. (2006)
5 more