› 1d85

X-ray diffraction
2.5Å resolution

STRUCTURAL CONSEQUENCES OF A CARCINOGENIC ALKYLATION LESION ON DNA: EFFECT OF O6-ETHYL-GUANINE ON THE MOLECULAR STRUCTURE OF D(CGC[E6G]AATTCGCG)-NETROPSIN COMPLEX

Released:
DOI: 10.2210/pdb1d85/pdb
PDB entry 1d85 coloured by chain, front view.
PDB entry 1d85 coloured by chain, side view.
PDB entry 1d85 coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Structural consequences of a carcinogenic alkylation lesion on DNA: effect of O6-ethylguanine on the molecular structure of the d(CGC[e6G]AATTCGCG)-netropsin complex.
Sriram M, van der Marel GA, Roelen HL, van Boom JH, Wang AH
Biochemistry 31 11823-34 (1992)
PMID: 1332773

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113642 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
DNA (5'-D(*CP*GP*CP*(G36)P*AP*AP*TP*TP*CP*GP*CP*G)-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 12 nucleotides
Theoretical weight: 3.69 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NT 1 x NT
1 modified residue:
Ligand G36 2 x G36

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 25.456Å b: 41.126Å c: 67.081Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 not available not available
Expression system: Not provided

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Citations

1 review citation

Crystal and solution structures of d(CGC[e6G]AATTCGCG)-drug complexes reveal conformational polymorphism of O6-ethyl-guanine:cytosine base pair.
Sriram et al. (1994)
38 other articles