› 1d8c

X-ray diffraction
2Å resolution

MALATE SYNTHASE G COMPLEXED WITH MAGNESIUM AND GLYOXYLATE

Released:
DOI: 10.2210/pdb1d8c/pdb
PDB entry 1d8c coloured by chain, front view.
PDB entry 1d8c coloured by chain, side view.
PDB entry 1d8c coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of Escherichia coli malate synthase G complexed with magnesium and glyoxylate at 2.0 A resolution: mechanistic implications.
Howard BR, Endrizzi JA, Remington SJ
Biochemistry 39 3156-68 (2000)
PMID: 10715138

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + H(2)O + glyoxylate = (S)-malate + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153394 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate synthase G Chain: A
Molecule details ›
Chain: A
Length: 723 amino acids
Theoretical weight: 81.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P37330 (Residues: 2-723; Coverage: 100%)
Gene names: JW2943, b2976, glc, glcB
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand SOR 1 x SOR
Ligand MG 1 x MG
Ligand SO4 5 x SO4
Ligand GLV 1 x GLV
1 modified residue:
Ligand MSE 23 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 73.8Å b: 88.7Å c: 109.9Å
α: 90° β: 90° γ: 90°
Expression system: Escherichia coli

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Citations

7 review citations

NMR studies of protein structure and dynamics.
Kay LE. (2005)
6 more

18 mentions without citation

Solution NMR-derived global fold of a monomeric 82-kDa enzyme.
Tugarinov et al. (2005)
17 more