› 1d8h

X-ray diffraction
2Å resolution

X-RAY CRYSTAL STRUCTURE OF YEAST RNA TRIPHOSPHATASE IN COMPLEX WITH SULFATE AND MANGANESE IONS.

Released:
DOI: 10.2210/pdb1d8h/pdb
PDB entry 1d8h coloured by chain, front view.
PDB entry 1d8h coloured by chain, side view.
PDB entry 1d8h coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure and mechanism of yeast RNA triphosphatase: an essential component of the mRNA capping apparatus.
Lima CD, Wang LK, Shuman S
Cell 99 533-43 (1999)
PMID: 10589681

Function and Biology Details

Reaction catalysed: 
A 5'-triphospho-[mRNA] + H(2)O = a 5'-diphospho-[mRNA] + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126743 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mRNA-capping enzyme subunit beta Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 311 amino acids
Theoretical weight: 35.69 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O13297 (Residues: 241-549; Coverage: 56%)
Gene names: CET1, P1433, YPL228W
Sequence domains: mRNA capping enzyme, beta chain
Structure domains: mRNA triphosphatase Cet1-like

Ligands and Environments

2 bound ligands:
Ligand MN 3 x MN
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 116.436Å b: 118.723Å c: 85.138Å
α: 90° β: 108.06° γ: 90°
R-values:
R R work R free
0.229 0.229 0.31
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

mRNA capping: biological functions and applications.
Ramanathan et al. (2016)
8 more

9 mentions without citation

Enzymology of RNA cap synthesis.
Ghosh et al. (2010)
8 more