› 1d8l

X-ray diffraction
2.5Å resolution

E. COLI HOLLIDAY JUNCTION BINDING PROTEIN RUVA NH2 REGION LACKING DOMAIN III

Released:
DOI: 10.2210/pdb1d8l/pdb
PDB entry 1d8l coloured by chain, front view.
PDB entry 1d8l coloured by chain, side view.
PDB entry 1d8l coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Modulation of RuvB function by the mobile domain III of the Holliday junction recognition protein RuvA.
Nishino T, Iwasaki H, Kataoka M, Ariyoshi M, Fujita T, Shinagawa H, Morikawa K
J Mol Biol 298 407-16 (2000)
PMID: 10772859

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141650 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Holliday junction branch migration complex subunit RuvA Chains: A, B
Molecule details ›
Chains: A, B
Length: 149 amino acids
Theoretical weight: 16.5 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A809 (Residues: 1-149; Coverage: 73%)
Gene names: JW1850, b1861, ruvA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P21212
Unit cell:
a: 79.6Å b: 115.6Å c: 34.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.243 0.32
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Holliday junction resolvases.
Wyatt et al. (2014)
3 more

1 mention without citation

Estimating loop length from CryoEM images at medium resolutions.
McKnight et al. (2013)