› 1d8v

Solution NMR

THE RESTRAINED AND MINIMIZED AVERAGE NMR STRUCTURE OF MAP30.

Released:
DOI: 10.2210/pdb1d8v/pdb
PDB entry 1d8v coloured by chain, front view.
PDB entry 1d8v coloured by chain, side view.
PDB entry 1d8v coloured by chain, top view.
Source organism: Momordica charantia
Primary publication:
Solution structure of anti-HIV-1 and anti-tumor protein MAP30: structural insights into its multiple functions.
Wang YX, Neamati N, Jacob J, Palmer I, Stahl SJ, Kaufman JD, Huang PL, Huang PL, Winslow HE, Pommier Y, Wingfield PT, Lee-Huang S, Bax A, Torchia DA
Cell 99 433-42 (1999)
PMID: 10571185

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150292 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosome-inactivating protein beta-momorcharin Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 29.63 KDa
Source organism: Momordica charantia
UniProt:
  • Canonical: P24817 (Residues: 24-286; Coverage: 100%)
Gene names: MAP30, RIP
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing

Quick links

Citations

10 review citations

Ribosome-inactivating proteins from plants: more than RNA N-glycosidases?
Peumans et al. (2001)
9 more

3 mentions without citation

Structures and Ribosomal Interaction of Ribosome-Inactivating Proteins.
Shi et al. (2016)
2 more