› 1daw

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF A BINARY COMPLEX OF PROTEIN KINASE CK2 (ALPHA-SUBUNIT) AND MG-AMPPNP

Released:
DOI: 10.2210/pdb1daw/pdb
PDB entry 1daw coloured by chain, front view.
PDB entry 1daw coloured by chain, side view.
PDB entry 1daw coloured by chain, top view.
Source organism: Zea mays
Primary publication:
GTP plus water mimic ATP in the active site of protein kinase CK2.
Niefind K, Pütter M, Guerra B, Issinger OG, Schomburg D
Nat Struct Biol 6 1100-3 (1999)
PMID: 10581548

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151243 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Casein kinase II subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 38.67 KDa
Source organism: Zea mays
Expression system: Escherichia coli
UniProt:
  • Canonical: P28523 (Residues: 2-328; Coverage: 99%)
Gene name: ACK2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: C2
Unit cell:
a: 143.11Å b: 59.2Å c: 45.81Å
α: 90° β: 103.56° γ: 90°
R-values:
R R work R free
0.223 0.212 0.285
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Protein kinase CK2: structure, regulation and role in cellular decisions of life and death.
Litchfield DW. (2003)
15 more

9 mentions without citation

Substrate and docking interactions in serine/threonine protein kinases.
Goldsmith et al. (2007)
8 more