› 1dbw

X-ray diffraction
1.6Å resolution

CRYSTAL STRUCTURE OF FIXJ-N

Released:
DOI: 10.2210/pdb1dbw/pdb
PDB entry 1dbw coloured by chain, front view.
PDB entry 1dbw coloured by chain, side view.
PDB entry 1dbw coloured by chain, top view.
Source organism: Sinorhizobium meliloti
Primary publication:
Structural transitions in the FixJ receiver domain.
Gouet P, Fabry B, Guillet V, Birck C, Mourey L, Kahn D, Samama JP
Structure 7 1517-26 (1999)
PMID: 10647182

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145541 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulatory protein FixJ Chains: A, B
Molecule details ›
Chains: A, B
Length: 126 amino acids
Theoretical weight: 13.88 KDa
Source organism: Sinorhizobium meliloti
Expression system: Escherichia coli
UniProt:
  • Canonical: P10958 (Residues: 1-126; Coverage: 62%)
Gene names: RA0669, SMa1227, fixJ
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand 15P 1 x 15P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32, ESRF BEAMLINE ID14-4
Spacegroup: P1
Unit cell:
a: 31.7Å b: 42.2Å c: 44.6Å
α: 93.2° β: 104.5° γ: 101.9°
R-values:
R R work R free
0.189 0.187 0.224
Expression system: Escherichia coli

Quick links

Citations

26 citation in other articles

Conformational changes in protein loops and helices induced by post-translational phosphorylation.
Groban et al. (2006)
25 more

8 mentions without citation

Allostery: absence of a change in shape does not imply that allostery is not at play.
Tsai et al. (2008)
7 more