› 1df0

X-ray diffraction
2.6Å resolution

Crystal structure of M-Calpain

Released:
DOI: 10.2210/pdb1df0/pdb
PDB entry 1df0 coloured by chain, front view.
PDB entry 1df0 coloured by chain, side view.
PDB entry 1df0 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystal structure of calpain reveals the structural basis for Ca(2+)-dependent protease activity and a novel mode of enzyme activation.
Hosfield CM, Elce JS, Davies PL, Jia Z
EMBO J 18 6880-9 (1999)
PMID: 10601010

Function and Biology Details

Reaction catalysed: 
Broad endopeptidase specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170434 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calpain-2 catalytic subunit Chain: A
Molecule details ›
Chain: A
Length: 700 amino acids
Theoretical weight: 79.99 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07009 (Residues: 1-700; Coverage: 100%)
Gene name: Capn2
Sequence domains:
Structure domains:
Calpain small subunit 1 Chain: B
Molecule details ›
Chain: B
Length: 184 amino acids
Theoretical weight: 21.3 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q64537 (Residues: 88-270; Coverage: 68%)
Gene names: Capn4, Capns1, Css1
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P1
Unit cell:
a: 57.696Å b: 80.181Å c: 80.72Å
α: 60.371° β: 70.848° γ: 79.486°
R-values:
R R work R free
0.223 0.223 0.293
Expression system: Escherichia coli

Quick links

Citations

59 review citations

The calpain system.
Goll et al. (2003)
58 more

10 mentions without citation

The calpains: modular designs and functional diversity.
Croall et al. (2007)
9 more