› 1dfa

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF PI-SCEI IN C2 SPACE GROUP

Released:
DOI: 10.2210/pdb1dfa/pdb
PDB entry 1dfa coloured by chain, front view.
PDB entry 1dfa coloured by chain, side view.
PDB entry 1dfa coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Probing the structure of the PI-SceI-DNA complex by affinity cleavage and affinity photocross-linking.
Hu D, Crist M, Duan X, Quiocho FA, Gimble FS
J Biol Chem 275 2705-12 (2000)
PMID: 10644733

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147903 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease PI-SceI Chain: A
Molecule details ›
Chain: A
Length: 454 amino acids
Theoretical weight: 51 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P17255 (Residues: 284-737; Coverage: 42%)
Gene names: CLS8, D1286, TFP1, VMA1, YDL185W
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 93.6Å b: 76Å c: 71.4Å
α: 90° β: 111.3° γ: 90°
R-values:
R R work R free
0.22 0.21 0.28
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility.
Chevalier et al. (2001)
2 more

4 mentions without citation

High resolution crystal structure of domain I of the Saccharomyces cerevisiae homing endonuclease PI-SceI.
Werner et al. (2002)
3 more