› 1dgc

X-ray diffraction
3Å resolution

THE X-RAY STRUCTURE OF THE GCN4-BZIP BOUND TO ATF/CREB SITE DNA SHOWS THE COMPLEX DEPENDS ON DNA FLEXIBILITY

Released:
DOI: 10.2210/pdb1dgc/pdb
PDB entry 1dgc coloured by chain, front view.
PDB entry 1dgc coloured by chain, side view.
PDB entry 1dgc coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The X-ray structure of the GCN4-bZIP bound to ATF/CREB site DNA shows the complex depends on DNA flexibility.
König P, Richmond TJ
J Mol Biol 233 139-54 (1993)
PMID: 8377181

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113678 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
General control transcription factor GCN4 Chain: A
Molecule details ›
Chain: A
Length: 62 amino acids
Theoretical weight: 7.24 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P03069 (Residues: 220-281; Coverage: 22%)
Gene names: AAS101, AAS3, ARG9, GCN4, YEL009C
Sequence domains: Basic region leucine zipper
Structure domains: Classic Zinc Finger
DNA (5'-D(*TP*GP*GP*AP*GP*AP*TP*GP*AP*CP*GP*TP*CP*AP*TP*CP*T P*CP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 19 nucleotides
Theoretical weight: 5.82 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P41212
Unit cell:
a: 58.66Å b: 58.66Å c: 86.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 not available
Expression system: Not provided

Quick links

Citations

16 review citations

Coiled coils: a highly versatile protein folding motif.
Burkhard et al. (2001)
15 more

3 mentions without citation

Gcn4-Mediator Specificity Is Mediated by a Large and Dynamic Fuzzy Protein-Protein Complex.
Tuttle et al. (2018)
2 more