› 1dgd

X-ray diffraction
2.8Å resolution

AN ALKALI METAL ION SIZE-DEPENDENT SWITCH IN THE ACTIVE SITE STRUCTURE OF DIALKYLGLYCINE DECARBOXYLASE

Released:
DOI: 10.2210/pdb1dgd/pdb
PDB entry 1dgd coloured by chain, front view.
PDB entry 1dgd coloured by chain, side view.
PDB entry 1dgd coloured by chain, top view.
Source organism: Burkholderia cepacia
Primary publication:
An alkali metal ion size-dependent switch in the active site structure of dialkylglycine decarboxylase.
Hohenester E, Keller JW, Jansonius JN
Biochemistry 33 13561-70 (1994)
PMID: 7947767

Function and Biology Details

Reaction catalysed: 
2,2-dialkylglycine + pyruvate = dialkyl ketone + CO(2) + L-alanine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147847 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2,2-dialkylglycine decarboxylase Chain: A
Molecule details ›
Chain: A
Length: 432 amino acids
Theoretical weight: 46.37 KDa
Source organism: Burkholderia cepacia
Expression system: Not provided
UniProt:
  • Canonical: P16932 (Residues: 2-433; Coverage: 100%)
Gene name: dgdA
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
3 bound ligands:
Ligand NA 1 x NA
Ligand LI 1 x LI
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P6422
Unit cell:
a: 152.7Å b: 152.7Å c: 86.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 not available not available
Expression system: Not provided

Quick links

Citations

6 review citations

Structure, evolution and action of vitamin B6-dependent enzymes.
Jansonius JN. (1998)
5 more

2 mentions without citation

Identifying unexpected therapeutic targets via chemical-protein interactome.
Yang et al. (2010)
1 more